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Genechem rna interference lentiviral vectors
Rna Interference Lentiviral Vectors, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+interference+lentiviral+vectors/interference+lentiviral+rna+vectors/pm41345704-74-4-18
Average 86 stars, based on 1 article reviews
rna interference lentiviral vectors - by Bioz Stars, 2026-10
86/100 stars

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Cell Culture:

Article Title: Glucose transporter 3 (GLUT3) promotes lactylation modifications by regulating lactate dehydrogenase A (LDHA) in gastric cancer.
Article Snippet: The concentration of the suspension was calculated by hemocytometer (Marienfeld, Germany), and appropriate cells were taken for further culture. .. AGS and HGC-27 cells (5 × 103) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. The lentiviral interference sequences are shown in Additional file 2: Table S1. ..

Article Title: BNIP3+ fibroblasts associated with hypoxia and inflammation predict prognosis and immunotherapy response in pancreatic ductal adenocarcinoma
Article Snippet: .. CAF were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in Additional file . ..

Article Title: BNIP3+ fibroblasts associated with hypoxia and inflammation predict prognosis and immunotherapy response in pancreatic ductal adenocarcinoma.
Article Snippet: .. CAF were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in Additional file 1. ..

Article Title: Overall survival prediction of gastric cancer using the gene signature of CT-detected extramural venous invasion combined with M2 macrophages infiltration.
Article Snippet: The cell lines were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin/streptomycin (Gibco, USA). .. HGC-27, KATO III, MKN-45 and MFC cells (5 × 103) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in additional file 2. ..

Article Title: Overall survival prediction of gastric cancer using the gene signature of CT-detected extramural venous invasion combined with M2 macrophages infiltration
Article Snippet: The cell lines were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin/streptomycin (Gibco, USA). .. HGC-27, KATO III, MKN-45 and MFC cells (5 × 10 3 ) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in additional file . ..

Article Title: Integration of bulk and single-cell transcriptomic data reveals a novel signature related to liver metastasis and basement membrane in pancreatic cancer
Article Snippet: .. CAFs were cultured in 24-well plates and transfected with previously RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24h. ..

Transfection:

Article Title: Glucose transporter 3 (GLUT3) promotes lactylation modifications by regulating lactate dehydrogenase A (LDHA) in gastric cancer.
Article Snippet: The concentration of the suspension was calculated by hemocytometer (Marienfeld, Germany), and appropriate cells were taken for further culture. .. AGS and HGC-27 cells (5 × 103) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. The lentiviral interference sequences are shown in Additional file 2: Table S1. ..

Article Title: BNIP3+ fibroblasts associated with hypoxia and inflammation predict prognosis and immunotherapy response in pancreatic ductal adenocarcinoma
Article Snippet: .. CAF were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in Additional file . ..

Article Title: BNIP3+ fibroblasts associated with hypoxia and inflammation predict prognosis and immunotherapy response in pancreatic ductal adenocarcinoma.
Article Snippet: .. CAF were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in Additional file 1. ..

Article Title: Overall survival prediction of gastric cancer using the gene signature of CT-detected extramural venous invasion combined with M2 macrophages infiltration.
Article Snippet: The cell lines were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin/streptomycin (Gibco, USA). .. HGC-27, KATO III, MKN-45 and MFC cells (5 × 103) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in additional file 2. ..

Article Title: Overall survival prediction of gastric cancer using the gene signature of CT-detected extramural venous invasion combined with M2 macrophages infiltration
Article Snippet: The cell lines were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin/streptomycin (Gibco, USA). .. HGC-27, KATO III, MKN-45 and MFC cells (5 × 10 3 ) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in additional file . ..

Article Title: Integration of bulk and single-cell transcriptomic data reveals a novel signature related to liver metastasis and basement membrane in pancreatic cancer
Article Snippet: .. CAFs were cultured in 24-well plates and transfected with previously RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24h. ..

Construct:

Article Title: Glucose transporter 3 (GLUT3) promotes lactylation modifications by regulating lactate dehydrogenase A (LDHA) in gastric cancer.
Article Snippet: The concentration of the suspension was calculated by hemocytometer (Marienfeld, Germany), and appropriate cells were taken for further culture. .. AGS and HGC-27 cells (5 × 103) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. The lentiviral interference sequences are shown in Additional file 2: Table S1. ..

Article Title: BNIP3+ fibroblasts associated with hypoxia and inflammation predict prognosis and immunotherapy response in pancreatic ductal adenocarcinoma
Article Snippet: .. CAF were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in Additional file . ..

Article Title: BNIP3+ fibroblasts associated with hypoxia and inflammation predict prognosis and immunotherapy response in pancreatic ductal adenocarcinoma.
Article Snippet: .. CAF were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in Additional file 1. ..

Article Title: Overall survival prediction of gastric cancer using the gene signature of CT-detected extramural venous invasion combined with M2 macrophages infiltration.
Article Snippet: The cell lines were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin/streptomycin (Gibco, USA). .. HGC-27, KATO III, MKN-45 and MFC cells (5 × 103) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in additional file 2. ..

Article Title: Overall survival prediction of gastric cancer using the gene signature of CT-detected extramural venous invasion combined with M2 macrophages infiltration
Article Snippet: The cell lines were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin/streptomycin (Gibco, USA). .. HGC-27, KATO III, MKN-45 and MFC cells (5 × 10 3 ) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in additional file . ..

Negative Control:

Article Title: Glucose transporter 3 (GLUT3) promotes lactylation modifications by regulating lactate dehydrogenase A (LDHA) in gastric cancer.
Article Snippet: The concentration of the suspension was calculated by hemocytometer (Marienfeld, Germany), and appropriate cells were taken for further culture. .. AGS and HGC-27 cells (5 × 103) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. The lentiviral interference sequences are shown in Additional file 2: Table S1. ..

Article Title: BNIP3+ fibroblasts associated with hypoxia and inflammation predict prognosis and immunotherapy response in pancreatic ductal adenocarcinoma
Article Snippet: .. CAF were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in Additional file . ..

Article Title: BNIP3+ fibroblasts associated with hypoxia and inflammation predict prognosis and immunotherapy response in pancreatic ductal adenocarcinoma.
Article Snippet: .. CAF were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in Additional file 1. ..

Article Title: Overall survival prediction of gastric cancer using the gene signature of CT-detected extramural venous invasion combined with M2 macrophages infiltration.
Article Snippet: The cell lines were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin/streptomycin (Gibco, USA). .. HGC-27, KATO III, MKN-45 and MFC cells (5 × 103) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in additional file 2. ..

Article Title: Overall survival prediction of gastric cancer using the gene signature of CT-detected extramural venous invasion combined with M2 macrophages infiltration
Article Snippet: The cell lines were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin/streptomycin (Gibco, USA). .. HGC-27, KATO III, MKN-45 and MFC cells (5 × 10 3 ) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in additional file . ..

Article Title: Integration of bulk and single-cell transcriptomic data reveals a novel signature related to liver metastasis and basement membrane in pancreatic cancer
Article Snippet: .. CAFs were cultured in 24-well plates and transfected with previously RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24h. ..

Plasmid Preparation:

Article Title: Glucose transporter 3 (GLUT3) promotes lactylation modifications by regulating lactate dehydrogenase A (LDHA) in gastric cancer.
Article Snippet: The concentration of the suspension was calculated by hemocytometer (Marienfeld, Germany), and appropriate cells were taken for further culture. .. AGS and HGC-27 cells (5 × 103) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. The lentiviral interference sequences are shown in Additional file 2: Table S1. ..

Article Title: BNIP3+ fibroblasts associated with hypoxia and inflammation predict prognosis and immunotherapy response in pancreatic ductal adenocarcinoma
Article Snippet: .. CAF were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in Additional file . ..

Article Title: BNIP3+ fibroblasts associated with hypoxia and inflammation predict prognosis and immunotherapy response in pancreatic ductal adenocarcinoma.
Article Snippet: .. CAF were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in Additional file 1. ..

Article Title: Overall survival prediction of gastric cancer using the gene signature of CT-detected extramural venous invasion combined with M2 macrophages infiltration.
Article Snippet: The cell lines were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin/streptomycin (Gibco, USA). .. HGC-27, KATO III, MKN-45 and MFC cells (5 × 103) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in additional file 2. ..

Article Title: Overall survival prediction of gastric cancer using the gene signature of CT-detected extramural venous invasion combined with M2 macrophages infiltration
Article Snippet: The cell lines were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin/streptomycin (Gibco, USA). .. HGC-27, KATO III, MKN-45 and MFC cells (5 × 10 3 ) were cultured in 24-well plates and transfected with previously constructed RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24 h. Interference sequences of lentiviral are shown in additional file . ..

Article Title: Integration of bulk and single-cell transcriptomic data reveals a novel signature related to liver metastasis and basement membrane in pancreatic cancer
Article Snippet: .. CAFs were cultured in 24-well plates and transfected with previously RNA interference lentiviral vectors (Genechem, China) or a negative control (empty plasmid) for 24h. ..

Generated:

Article Title: Lactylation-driven KRT19 promotes non-small cell lung cancer progression by suppressing cellular senescence.
Article Snippet: .. Lentiviral vectors, Plasmids and RNA interference Lentiviral vectors encoding Flag-tagged KRT19 or shRNAs targeting KRT19 were generated by GeneChem (Shanghai, China). ..

other:

Article Title: Construction of a Novel Mitochondria-Associated Gene Model for Assessing ESCC Immune Microenvironment and Predicting Survival.
Article Snippet: Department of General Practice, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, P.R.. China 2 Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, P.R.. China School of Foreign Languages, Shandong University of Finance and Economics, Jinan 250014, P. R. China Department of Surgical, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, P.R.



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